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Genome Editing of MSCs as a Platform for Cell Therapy

  • Krissanapong Manotham
  • Supreecha Chattong
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1867)

Abstract

The rapid growing of genome editing technology leads to an optimistic expectation for the treatment of many incurable diseases. The core of genome editing relies on DNA-repairing processes which occur independently in each cell, thus generating a mix of genetic variation cells. Here, we describe the protocol of using mesenchymal stem cells as a platform to generate high purity of ZFN-edited patients’ cell clones which may be useful in conjunction with therapeutic cell conversion and reprograming.

Key words

Genome editing Zinc-finger nuclease (ZFN) Mesenchymal stem cells Homologous recombination 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Krissanapong Manotham
    • 1
  • Supreecha Chattong
    • 1
    • 2
  1. 1.Molecular and Cellular Biology Unit, Department of MedicineLerdsin General HospitalBangkokThailand
  2. 2.EST Laboratory, S.S. ManufacturingNonthaburiThailand

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