Efficient and Fast Production of Transgenic Rice Plants by Agrobacterium-Mediated Transformation
Genetic transformation plays a key role in deciphering regulation of agronomic traits at molecular level in rice, a model monocot cereal crop. Here we describe an efficient and fast protocol for producing transgenic japonica rice plants using the Agrobacterium-mediated transformation method. The protocol simplifies medium compositions and transformation steps and can be easily followed by a lab technician with little tissue culture experience. Using this protocol, we have transformed thousands of gene constructs in the past 10 years and edited hundreds of genes with the CRISPR-Cas9 system recently.
Key wordsRice Agrobacterium-mediated transformation Callus induction Selection of transformants Plant regeneration
This work was supported by the Innovation Program of Chinese Academy of Agricultural Sciences.
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