Setaria viridis as a Model Plant for Functional Genomic Studies in C4 Crops

  • Polyana Kelly Martins
  • Bárbara Andrade Dias Brito da Cunha
  • Adilson Kenji Kobayshi
  • Hugo Bruno Correa MolinariEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1864)


Setaria viridis is an emerging model for C4 species, and it is an important model to validate some genes for further C4 crop transformation, such as sugarcane, maize, and wheat. Here, we describe two protocols for stable transformation of S. viridis mediated by Agrobacterium tumefaciens with three different reporter genes and two selectable markers. Routine transformation efficiency reaching 29% was achieved using embryogenic callus in S. viridis (accession A10.1). Alternatively, we developed a transformation method by floral dip with 0.6% efficiency. The developed protocols could be useful for genetic and genomics studies of important food-feed-fiber-fuel C4 crops.

Key words

Embryogenic callus Floral dip Transformation Agrobacterium Grasses 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Polyana Kelly Martins
    • 1
  • Bárbara Andrade Dias Brito da Cunha
    • 1
  • Adilson Kenji Kobayshi
    • 1
  • Hugo Bruno Correa Molinari
    • 1
    Email author
  1. 1.Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE), Brazilian Agricultural Research Corporation (EMBRAPA), Parque Estação BiológicaBrasíliaBrazil

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