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Agrobacterium tumefaciens-Mediated Transformation of Tomato

  • Joyce Van Eck
  • Patricia Keen
  • Michelle Tjahjadi
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1864)

Abstract

Tomato is both an important food crop and serves as a model plant species that is used for various research investigations including understanding gene function. Transformation is commonly utilized to facilitate these investigations in combination with all the extensive genetic and genomic resources available for tomato. The transformation protocol routinely used in our laboratory has been applied to many different tomato genotypes and relies on Agrobacterium tumefaciens infection of young cotyledon sections. We have used vector systems for overexpression, RNA interference for gene silencing, and CRISPR/Cas9 for genome editing. Vectors used to design gene constructs contained selectable marker genes that conferred resistance to kanamycin, hygromycin, and the herbicide component, bialaphos. The protocol we follow for Agrobacterium-mediated transformation of both cultivated and wild species of tomato is detailed in this chapter.

Key words

AGL1 Agrobacterium tumefaciens LBA4404 Solanaceae Solanum lycopersicum Solanum pimpinellifolium 

Notes

Acknowledgments

Van Eck acknowledges the National Science Foundation Plant Genome Research Program (IOS-1732253) for support related to her research on genetic engineering and genome editing of tomato.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Joyce Van Eck
    • 1
    • 2
  • Patricia Keen
    • 1
  • Michelle Tjahjadi
    • 1
  1. 1.The Boyce Thompson InstituteIthacaUSA
  2. 2.Plant Breeding and Genetics SectionSchool of Integrative Plant Science, Cornell UniversityIthacaUSA

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