Measuring Rates of ATP Synthesis
Here, we offer you a high-throughput assay to measure the ATP synthesis capacity in cells or isolated mitochondria. More specifically, the assay is linked to the mitochondrial’ electron transport chain components of your interest being either through complex I (with or without a linkage to pyruvate dehydrogenase activity), through complex II, or through the electron transport flavoprotein and complex I (β-oxidation of fatty acids).
Key wordsATP synthesis Oxidative phosphorylation β-oxidation Mitochondria Bioenergetics Oxidative phosphorylation (OXPHOS)
DC and PV are senior clinical investigators of the Research Foundation-Flanders (FWO-Belgium).
The authors declare no competing interests.