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High-Throughput Measure of Bioactive Lipids Using Non-targeted Mass Spectrometry

  • Kim A. Lagerborg
  • Jeramie D. Watrous
  • Susan Cheng
  • Mohit Jain
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1862)

Abstract

Bioactive lipids represent critical intra- and intercellular signaling molecules, and have been implicated in both physiologic homeostasis and disease pathology. Measurement of bioactive lipids is vital toward understanding the role of these signaling intermediates in human biology. Current analytical methods for assessment of bioactive lipids in human biosamples are limited, however, in breath of analytes assayed as well as robustness and time required for measures across thousands of samples. Herein, we describe in comprehensive detail a rapid and robust analytical method using liquid chromatography-mass spectrometry (LC-MS) for non-targeted measurement of over 7000 bioactive lipids, including eicosanoids and eicosanoid-related metabolites, in human biosamples. These methods may be applied to the study of population scale cohorts to uncover previously unrecognized roles for bioactive lipid species in human biology.

Key words

Bioactive lipids Eicosanoids Mass spectrometry Lipidomics LC-MS 

Notes

Acknowledgments

This work was supported by grants from the UC San Diego Frontiers of Innovation Scholars Program (K.L.), the Doris Duke Charitable Foundation (#2015092: M.J.), Tobacco-Related Disease Research Program (#24RT-0032: M.J., #24FT-0010: J.D.W.), the American Heart Association (CVGPS Pathway Award: S.C., M.J.), and National Institutes of Health (R01ES027595; R01HL134168: M.J., S.C.).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Kim A. Lagerborg
    • 1
    • 2
  • Jeramie D. Watrous
    • 1
    • 2
  • Susan Cheng
    • 3
    • 4
  • Mohit Jain
    • 1
    • 2
  1. 1.Department of MedicineUniversity of California, San DiegoLa JollaUSA
  2. 2.Department of PharmacologyUniversity of California, San DiegoLa JollaUSA
  3. 3.Cardiovascular Division, Department of Medicine, Brigham and Women’s HospitalHarvard Medical SchoolBostonUSA
  4. 4.Framingham Heart StudyFraminghamUSA

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