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Live Imaging of Xist RNA

  • Osamu Masui
  • Edith Heard
  • Haruhiko Koseki
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1861)

Abstract

Live imaging gives additional layers of information such as physical dynamics of a molecule of your interest. Aptamer-based green fluorescent protein (GFP) labeling is suitable for visualization of RNA molecules. Here we describe a method to visualize Xist RNA using the Bgl aptamer system.

Key words

Live imaging Aptamer Xist RNA Bgl system 

Notes

Acknowledgments

I thank Drs. Edith Heard and Haruhiko Koseki for critical discussions. This work was supported by PRESTO and KAKENHI to O.M.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratory for Developmental Genetics, Center for Integrative Medical SciencesRIKEN Yokohama InstituteYokohamaJapan
  2. 2.Mammalian Developmental Epigenetics Group, Institut CuriePSL University, CNRS UMR3215, INSERM U934ParisFrance

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