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Somatic Cell Nuclear Transfer in Mice: Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status

  • Kimiko Inoue
  • Shogo Matoba
  • Atsuo Ogura
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1861)

Abstract

Somatic cell nuclear transfer (SCNT) enables the production of animals from single cell nuclei. Unlike normally fertilized embryos, SCNT-derived embryos ectopically express the Xist gene from the maternal allele, because of the lack of Xist-repressing imprints in the somatic donor genome. This has severely compromised the development of SCNT-derived embryos, at least in mice. Here, we describe the basic protocol of mouse SCNT as well as a Xist knockdown SCNT procedure, which remarkably increases the efficiency of cloning mice.

Key words

Development Oocyte Xist Preimplantation embryo 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.RIKEN BioResource Research CenterTsukubaJapan

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