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Application of Heat to Quickly Stain and Destain Proteins Stained with Coomassie Blue

  • Biji T. Kurien
  • R. Hal Scofield
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1853)

Abstract

Proteins separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis have been visualized reliably by staining with Coomassie Brilliant Blue. In this chapter, we show that it is possible to drastically reduce protein staining and destaining time, while simultaneously increasing detection sensitivity, with the application of heat. It took 5 min to stain proteins at 55, 62.5, or 70 °C for a 1.5 mm gel, while it took 45, 45, and 20 min respectively for destaining. The time for staining was 1 min for a 0.8 mm gel at 65 °C, 2 min at 60 °C and 5 min at 55 °C. The destaining of proteins separated on a 0.8 mm gel took 8, 15, and 20 min at 65, 60, and 55 °C respectively. Proteins can be stained and destained rapidly with the use of heat, while enhancing detection sensitivity.

Key words

SDS-PAGE Coomassie Brilliant Blue Heat Destaining 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Section of Endocrinology and DiabetesUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA
  2. 2.Department of Veterans Affairs Medical CenterOklahoma CityUSA
  3. 3.Department of Arthritis and Clinical ImmunologyOklahoma CityUSA

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