Radiolabeling and Analysis of Labeled Proteins
Protein-based preparations for biomedical research, and radiolabeled pharmaceuticals for nuclear medical diagnostics and therapy require highest chemical, radiochemical, and in particular protein purity. For detection of contaminations most commonly size exclusion high-performance liquid chromatography and polyacrylamide gel analysis in combination with silver staining is used. Here we describe a by far more sensitive radiolabeling method for the detection of traces of contaminating proteins.
Key wordsAutoradiography Polyacrylamide gel Proteins
- 1.Albert S, Arndt C, Feldmann A, Bergmann R, Bachmann D, Koristka S, Ludwig F, Ziller-Walter P, Kegler A, Gärtner S, Schmitz M, Ehninger A, Cartellieri M, Ehninger G, Pietzsch J, Steinbach J, Bachmann M (2017) A novel nanobody-based target module for retargeting of T lymphocytes to EGFR-expressing cancer cells via the modular UniCAR platform. Oncoimmunol. https://doi.org/10.1080/2162402X.2017.128724