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Radiolabeling and Analysis of Labeled Proteins

  • Nicole Berndt
  • Ralf Bergmann
  • Michael Bachmann
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1853)

Abstract

Protein-based preparations for biomedical research, and radiolabeled pharmaceuticals for nuclear medical diagnostics and therapy require highest chemical, radiochemical, and in particular protein purity. For detection of contaminations most commonly size exclusion high-performance liquid chromatography and polyacrylamide gel analysis in combination with silver staining is used. Here we describe a by far more sensitive radiolabeling method for the detection of traces of contaminating proteins.

Key words

Autoradiography Polyacrylamide gel Proteins 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Nicole Berndt
    • 1
  • Ralf Bergmann
    • 1
  • Michael Bachmann
    • 1
  1. 1.Institute of Radiopharmaceutical Cancer Research, Radioimmunology, Helmholtz-Zentrum Dresden-Rossendorf e.V.(HZDR)DresdenGermany

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