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Multicolored Prestained Standard Protein Marker Generation Using a Variety of Remazol Dyes for Easy Visualization of Protein Bands During SDS-PAGE

  • Gaurav Kumar
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1853)

Abstract

Isolation and detection of a specific protein from a complex mixture of proteins using molecular sieves provided by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique in molecular biology and biochemistry. The detection is achieved usually by running prestained standard markers along with the sample protein mixture. Multicolored standard markers are preferred compared to fluorescent labeled or single colored markers as it allows easy visualization of separation of a specific protein band during SDS-PAGE. In addition, these colored markers show evidence for the transfer of protein bands on the membrane in western blotting technique. This review describes a very simple, inexpensive and easy method of prestaining specific proteins with different colors using a variety of Remazol dyes.

Key words

Sodium dodecyl sulfate–polyacrylamide gel electrophoresis Colored prestained markers Standard marker proteins Remazol dyes 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Oklahoma Medical Research Foundation, University of OklahomaOklahoma CityUSA

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