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Fungal Laccase Efficiently Destains Coomassie Brilliant Blue-R-250 Stained Polyacrylamide Gels

  • Gaurav Kumar
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1853)

Abstract

Fungal laccase-mediated destaining of Coomassie Brilliant Blue-R-250 stained polyacrylamide electrophoretic gel is very sensitive and environment friendly. Fermenting of Cerrena sp. HYB07 yields a broth with laccase activity which when supplemented with distilled water and used as a destaining solution results in a clear gel background in 2 h at 37 °C. Also, separation of the laccase enzyme for efficient functioning is not required. The method utilizes gel destaining and process of decolorizing dye simultaneously with a detection sensitivity of up to 10 ng. This technique avoids the use of hazardous organic compounds, as the destaining solution and the destain solution could be discarded easily without any further treatment.

Key words

Fungal laccase Destaining of gel Sodium dodecyl sulfate–polyacrylamide gel electrophoresis Coomassie Brilliant Blue R-250 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Oklahoma Medical Research Foundation, University of OklahomaOklahoma CityUSA

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