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In-Gel Protein Phosphatase Assay Using Fluorogenic Substrates

  • Isamu Kameshita
  • Noriyuki Sueyoshi
  • Atsuhiko Ishida
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1853)

Abstract

Protein phosphorylation plays important roles in regulating a variety of biological processes in animals, plants, and microorganisms. Therefore, it is important to use appropriate techniques to detect and analyze protein kinases and protein phosphatases. In this chapter, we describe the method to detect protein phosphatase activities using fluorogenic substrates such as 4-methylumbelliferyl phosphate (MUP) after separating proteins by one-dimensional or two-dimensional polyacrylamide gel electrophoresis.

Key words

In-gel assay Protein phosphatase Fluorogenic substrate Polyacrylamide gel electrophoresis 

Notes

Acknowledgments

This work was supported in part by the JSPS Grant-in-Aid for Scientific Research.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Isamu Kameshita
    • 1
  • Noriyuki Sueyoshi
    • 1
  • Atsuhiko Ishida
    • 2
  1. 1.Faculty of Agriculture, Kagawa UniversityMiki-choJapan
  2. 2.Graduate School of Integrated Arts and SciencesHiroshima UniversityHigashi-HiroshimaJapan

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