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A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins

  • Jelena Bogdanović Pristov
  • Ivan Spasojević
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1853)

Abstract

Native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS)–PAGE are among the most frequently applied techniques in protein analysis. Here we describe a fast one-step method for fluorescent visualization of proteins. Following PAGE, gels are soaked in solution of potassium ferricyanide (100 mM) in 1 M NaOH, and are kept in the dark for 30 min. Gels are then transferred to water and scanned. The sensitivity of the method is comparable with standard Coomassie Brilliant Blue staining.

Key words

Electrophoresis PAGE Staining protocol Fluorescence Tryptophan Ferricyanide 

Notes

Acknowledgments

This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia; Grant number OI173017.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Jelena Bogdanović Pristov
    • 1
  • Ivan Spasojević
    • 1
  1. 1.Department of Life Sciences, Institute for Multidisciplinary ResearchUniversity of BelgradeBelgradeSerbia

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