A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins
Native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS)–PAGE are among the most frequently applied techniques in protein analysis. Here we describe a fast one-step method for fluorescent visualization of proteins. Following PAGE, gels are soaked in solution of potassium ferricyanide (100 mM) in 1 M NaOH, and are kept in the dark for 30 min. Gels are then transferred to water and scanned. The sensitivity of the method is comparable with standard Coomassie Brilliant Blue staining.
Key wordsElectrophoresis PAGE Staining protocol Fluorescence Tryptophan Ferricyanide
This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia; Grant number OI173017.
- 3.Kurien BT, Scofield RH (1998) Heat mediated quick Coomassie blue staining and destaining of SDS–PAGE gels. Ind J Biochem Biophys 35:385–389Google Scholar