TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots After PAGE
Polyacrylamide gel electrophoresis (PAGE) has become one of the most powerful and widely used separation techniques for complex biological samples, whose traditional detection methods include organic dye or silver staining. For simple, convenient, and ultrasensitive detection of proteins after PAGE, a novel enhanced photoluminescent (PL) imaging method was developed. Thioglycolic acid (TGA)-capped CdTe quantum dots (QDs) and the enhancer reagent tetramethylethylenediamine (TEMED) were introduced, achieving the direct detection of various proteins in native 1-DE, 2-DE and SDS-PAGE. Here we describe the general protocol of TEMED enhanced PL imaging by QDs, including materials, practical procedures, and some notes.
Key wordsCdTe Quantum dots TEMED Photoluminescent imaging Polyacrylamide gel electrophoresis Human serum proteins
J. Ouyang thanks the financial support by the National Nature Science Foundation of China (21475011 and 21675014) and National Grant of Basic Research Program of China (2011CB915504). N. Na thanks the financial support provided by the National Nature Science Foundation of China (214220503 and 21675015) and the Fundamental Research Funds for the Central Universities.
- 16.Kedarnath G, Kumbhare LB, Jain VK, Phadnis PP, Nethaji M (2006) Group 12 metal monoselenocarboxylates: synthesis, characterization, structure and their transformation to metal selenide (MSe; M = Zn, Cd, Hg) nanoparticles. Dalton Trans:2714–2718Google Scholar
- 17.Jun YW, Koo JE, Cheon J (2000) One-step synthesis of size tuned zinc selenide quantum dots via a temperature controlled molecular precursor approach. Chem Commun:1243–1244Google Scholar