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Fluorescent Staining of Gels

  • Engelbert Buxbaum
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1853)

Abstract

Certain transition metal complexes show intensive fluorescence when bound to proteins. They can be used to stain gels after electrophoresis with a sensitivity approaching that of silver staining, but in a much simpler and more reproducible procedure. Stains can be prepared easily and at a fraction of the cost of commercially available reagents. Hydrophobic dyes can be used to stain gels without fixing; they do not interfere with later blotting or electroelution.

Key words

SDS-PAGE Fluorescent gel staining Ruthenium(II)tris(bathophen-anthroline disulfonate) RuBPS Nile red 

Notes

Acknowledgments

This work was supported by funding from Ross University.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Engelbert Buxbaum
    • 1
  1. 1.KevelaerGermany

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