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Considerations in the Use of Codon Optimization for Recombinant Protein Expression

  • Vincent P. MauroEmail author
  • Stephen A. Chappell
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1850)

Abstract

Codon optimization is a gene engineering approach that is commonly used for enhancing recombinant protein expression. This approach is possible because (1) degeneracy of the genetic code enables most amino acids to be encoded by multiple codons and (2) different mRNAs encoding the same protein can vary dramatically in the amount of protein expressed. However, because codon optimization potentially disrupts overlapping information encoded in mRNA coding regions, protein structure and function may be altered. This chapter discusses the use of codon optimization for various applications in mammalian cells as well as potential consequences, so that informed decisions can be made on the appropriateness of using this approach in each case.

Key words

Codon Optimization Synonymous mRNA Translation Wobble Recombinant Bioproduction Nucleic acid Therapeutics 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.The Scripps Research InstituteLa JollaUSA
  2. 2.LeidosSan DiegoUSA

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