Isolating and Characterizing Adipose-Derived Stem Cells

  • Guangpeng Liu
  • Xi Chen
Part of the Methods in Molecular Biology book series (MIMB, volume 1842)


The research and application of regeneration medicine will require a reliable source of stem cells. Adipose tissue has proven to be an easily accessible and rich source of adult stem cells, termed adipose-derived stem cells (ASCs). ASCs have the most important advantage over stem cells from other available sources. There is no other human tissue as abundant as adipose tissue, making it possible to isolate adequate numbers of ASCs for potential clinical applications. Here, we describe detailed methods for isolating and characterizing ASCs. These procedures can be applied to adipose tissue not only for humans but also for other species.

Key words

Adipose tissue Adult stem cells Multilineage differentiation Isolation Characterization 



This work was financially supported by the Natural Science Foundation of China (Research number 31271027 and 81171475).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Guangpeng Liu
    • 1
  • Xi Chen
    • 1
  1. 1.Department of Plastic and Reconstructive Surgery, Shanghai Tenth People’s HospitalTongJi University School of MedicineShanghaiPeople’s Republic of China

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