Protein Enrichment from Highly Dilute Samples with StrataClean

  • Florian BonnEmail author
  • Andreas Otto
Part of the Methods in Molecular Biology book series (MIMB, volume 1841)


Efficient and reproducible protein enrichment is a prerequisite for the proteomic analysis of highly dilute solutions like culture supernatant or body fluids. Traditionally several precipitation strategies were used for this purpose, but they have certain drawbacks. Here, a protocol for StrataClean enrichment—a very efficient and easy-to-use method for the enrichment of proteins from highly dilute samples—which is compatible to gel-free proteomics, is presented.

Key words

Protein enrichment StrataClean Solid-phase enrichment Highly dilute samples 



The authors like to thank Jürgen Bartel and Knut Büttner for their support in developing of the protocol. This work was supported by grants from the Deutsche Forschungsgemeinschaft (Transregio 34 Pathophysiology of staphylococci in the post-genomic era).


  1. 1.
    Chevallet M, Diemer H, Van Dorssealer A et al (2007) Toward a better analysis of secreted proteins: the example of the myeloid cells secretome. Proteomics 7(11):1757–1770CrossRefPubMedCentralPubMedGoogle Scholar
  2. 2.
    McCormick RM (1989) A solid-phase extraction procedure for DNA purification. Anal Biochem 181(1):66–74CrossRefPubMedCentralPubMedGoogle Scholar
  3. 3.
    Zanivan S, Maione F, Hein MY et al (2013) SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor Angiogenic markers. Mol Cell Proteomics 12(12):3599–3611CrossRefPubMedCentralPubMedGoogle Scholar
  4. 4.
    Pasztor L, Ziebandt A, Nega M et al (2010) Staphylococcal major autolysin (Atl) is involved in excretion of cytoplasmic proteins. J Biol Chem 285(47):36794–36803CrossRefPubMedCentralPubMedGoogle Scholar
  5. 5.
    Bonn F, Bartel J, Büttner K et al (2014) Picking vanished proteins from the void: how to collect and ship/share extremely dilute proteins in a reproducible and highly efficient manner. Anal Chem 86(15):7421–7427CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Biochemistry II, University HospitalGoethe University School of MedicineFrankfurtGermany
  2. 2.Department of Microbial Proteomics, Institute for MicrobiologyUniversity GreifswaldGreifswaldGermany

Personalised recommendations