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Functional Genomics and Seed Development in Medicago truncatula: An Overview

  • Christine Le Signor
  • Vanessa Vernoud
  • Mélanie Noguero
  • Karine Gallardo
  • Richard D. Thompson
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1822)

Abstract

The study of seed development in the model species Medicago truncatula has made a significant contribution to our understanding of this process in crop legumes. Thanks to the availability of comprehensive proteomics and transcriptomics databases, coupled with exhaustive mutant collections, the roles of several regulatory genes in development and maturation are beginning to be deciphered and functionally validated. Advances in next-generation sequencing and the availability of a genomic sequence have made feasible high-density SNP genotyping, allowing the identification of markers tightly linked to traits of agronomic interest. A further major advance is to be expected from the integration of omics resources in functional network construction, which has been used recently to identify “hub” genes central to important traits.

Keywords

Desiccation tolerance Longevity Globulin GWAS Network Dormancy Flavonoid AFL 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Christine Le Signor
    • 1
  • Vanessa Vernoud
    • 1
  • Mélanie Noguero
    • 1
  • Karine Gallardo
    • 1
  • Richard D. Thompson
    • 1
  1. 1.Agroécologie, AgroSup DijonINRA, Univ. Bourgogne Franche-ComtéF-21000 DijonFrance

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