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Method for Detection of miRNAs in Non-Model Organisms with Unreported Database

  • Parveen Bansal
  • Ashish Kumar
  • Sudhir Chandna
  • Malika Arora
  • Renu Bansal
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1823)

Abstract

Non-model organisms are studied very frequently, as a simple accessible and convenient system to investigate the role of miRNAs in particular aspect of biology or disease. However, the unavailability of the annotated genome and hence miRNA database of these non-model organisms pose a major constraint for using them more efficiently. Here, we describe a new method to identify miRNAs in non-model organisms without complex sequencing strategies and using miRNAs from close relative organisms as proxy/reference sequences.

Keywords

miRNA Non-model organisms 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Parveen Bansal
    • 1
  • Ashish Kumar
    • 2
  • Sudhir Chandna
    • 3
  • Malika Arora
    • 4
  • Renu Bansal
    • 5
  1. 1.University Centre of Excellence in Research, Baba Farid University of Health SciencesFaridkotIndia
  2. 2.Texas Biomedical Research InstituteSan AntonioUSA
  3. 3.Division of Natural Radiation Response MechanismsInstitute of Nuclear Medicine and Allied SciencesTimarpurIndia
  4. 4.Multidisciplinary Research Unit, Indian Council of Medical Research, GGSMCHFaridkotIndia
  5. 5.Department of MicrobiologyGGSMCHFaridkotIndia

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