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Targeting miRNA for Therapeutics Using a Micronome Based Method for Identification of miRNA-mRNA Pairs and Validation of Key Regulator miRNA

  • Parveen Bansal
  • Ashish Kumar
  • Sudhir Chandna
  • Malika Arora
  • Renu Bansal
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1823)

Abstract

MicroRNAs are 18–22 bp long non-coding sequences and play a critical role in diverse biological processes, through modulation of gene expression at the post-transcriptional level by binding at the 3′-untranslated region of target mRNA. Consequent upon the discovery of structural and functional features of miRNA targeting, several molecular methods have been developed to identify miRNA targets. However, these methods suffer several drawbacks, including technical challenges, requirement of high cell volumes, inability to differentiate between direct and indirect targets, cell/tissue as well as experimental-specificity and imprecise binding site information. Alternatively in silico approach enables the exploration of the potential miRNA-mRNA pairs to investigate signature miRNA and proteins involved in the signaling of various diseases. Here, we describe micronome-based standard method for identification of miRNA-mRNA pairs as well as validation of key regulator miRNA.

Keywords

MicroRNA Search Tool for Retrieval of Interacting Genes/Proteins (STRING) miRNA Target Protein–protein Interaction RNase protection assay mirTARbase 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Parveen Bansal
    • 1
  • Ashish Kumar
    • 2
  • Sudhir Chandna
    • 3
  • Malika Arora
    • 4
  • Renu Bansal
    • 5
  1. 1.University Centre of Excellence in ResearchBaba Farid University of Health SciencesFaridkotIndia
  2. 2.Texas Biomedical Research InstituteSan AntonioUSA
  3. 3.Division of Natural Radiation Response MechanismsInstitute of Nuclear Medicine and Allied SciencesTimarpurIndia
  4. 4.Multidisciplinary Research Unit, Indian Council of Medical ResearchGGSMCHFaridkotIndia
  5. 5.Department of MicrobiologyGGSMCHFaridkotIndia

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