Micropropagation in the Twenty-First Century

  • Jean Carlos Cardoso
  • Lee Tseng Sheng Gerald
  • Jaime A. Teixeira da Silva
Part of the Methods in Molecular Biology book series (MIMB, volume 1815)


Despite more than a century of research on effective biotechnological methods, micropropagation continues to be an important tool for the large-scale production of clonal plantlets of several important plant species that retain genetic fidelity and are pest-free. In some cases, micropropagation is the only technique that supports the maintenance and promotes the economic value of specific agricultural species. The micropropagation of plants solved many phytosanitary problems and allowed both the expansion and access to high-quality plants for growers from different countries and economic backgrounds, thereby effectively contributing to an agricultural expansion in this and the last century. The challenges for micropropagation in the twenty-first century include cost reduction, enhanced efficiency, developing new technologies, and combining micropropagation with other systems/propagation techniques such as microcuttings, hydroponics, and aeroponics. In this chapter, we discuss the actual uses of micropropagation in this century, its importance and limitations, and some possible techniques that can effectively increase its wider application by replacing certain conventional techniques and technologies.

Key words

Actual applications Breeding Combined techniques Cost reduction Micropropagation Plant tissue culture Large-scale production Secondary metabolic production Somatic embryogenesis 



JCC thanks CNPQ for the research fellowship No. 304174/2015-7.

Contribution Statements

LTSG contributed writing item 2.4 Bioreactors Systems to Reduce the Cost of Micropropagated Plantlets. JATS contributed writing items 2.1 LEDs, CCFLs, and Laser Light for Tissue Culture and 3.2 The Use of Micropropagation for Secondary Metabolite Production and with final revision of all the text. JCC contributed writing all other items in the chapter and final revision of the text.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Jean Carlos Cardoso
    • 1
  • Lee Tseng Sheng Gerald
    • 1
  • Jaime A. Teixeira da Silva
    • 2
  1. 1.Laboratory of Plant Physiology and Tissue Culture, Department of Biotechnology, Plant and Animal Production, Centro de Ciências AgráriasUniversidade Federal de São CarlosArarasBrazil
  2. 2.KagawaJapan

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