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Methods to Study TCDD-Inducible Poly-ADP-Ribose Polymerase (TIPARP) Mono-ADP-Ribosyltransferase Activity

  • David Hutin
  • Giulia Grimaldi
  • Jason Matthews
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1813)

Abstract

TCDD-inducible poly-ADP-ribose polymerase (TIPARP; also known as PARP7 and ARTD14) is a mono-ADP-ribosyltransferase that has emerged as an important regulator of innate immunity, stem cell pluripotency, and transcription factor regulation. Characterizing TIPARP’s catalytic activity and identifying its target proteins are critical to understanding its cellular function. Here we describe methods that we use to characterize TIPARP catalytic activity and its mono-ADP-ribosylation of its target proteins.

Key words

Immunoprecipitation TCDD-inducible poly-ADP-ribose polymerase (TIPARP) Protein purification Biotinylated-NAD+ 32P-NAD+ 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Pharmacology and ToxicologyUniversity of TorontoTorontoCanada
  2. 2.Department of Nutrition, Institute of Basic Medical SciencesUniversity of OsloOsloNorway

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