Hydrolysis of ADP-Ribosylation by Macrodomains

  • Melanija Posavec Marjanovic´
  • Gytis Jankevicius
  • Ivan AhelEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1813)


ADP-ribosylation is the process of transferring the ADP-ribose moiety from NAD+ to a substrate. While a number of proteins represent well described substrates accepting ADP-ribose modification, a recent report demonstrated biological role for DNA ADP-ribosylation as well. The conserved macrodomain fold of several known hydrolyses was found to possess de-ADP-ribosylating activity and the ability to hydrolyze (reverse) ADP-ribosylation. Here we summarize the methods that can be employed to study mono-ADP-ribosylation hydrolysis by macrodomains.

Key words

Macrodomain fold ADP-ribosylation Hydrolysis Toxin-antitoxin system NAD+ 



The work in Ahel lab is supported by Wellcome Trust (grant number 101794), the European Research Council (grant number 281739) and by Cancer Research UK (grant number C35050/A22284). MPM is financed by Croatian National Centre of Research Excellence in Personalized Healthcare grant.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Melanija Posavec Marjanovic´
    • 1
  • Gytis Jankevicius
    • 2
  • Ivan Ahel
    • 2
    Email author
  1. 1.University of ZagrebZagrebCroatia
  2. 2.Sir William Dunn School of PathologyUniversity of OxfordOxfordUK

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