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Monitoring Expression and Enzyme Activity of Ecto-ARTCs

  • Stephan Menzel
  • Sahil Adriouch
  • Peter Bannas
  • Friedrich Haag
  • Friedrich Koch-Nolte
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1813)

Abstract

Mammalian ARTCs are expressed as glycosylphosphatidylinositol (GPI)-anchored ectoenzymes (ARTC1–ARTC4) or secretory proteins (ARTC5) by different cell types. The ARTC2 enzymes catalyze mono-ADP-ribosylation of arginine residues in the extracellular domain of membrane proteins or secretory proteins. In this chapter we provide protocols to monitor the expression and activity of ARTCs on the cell membrane of living cells and in soluble form in biological fluids.

Key words

ADP-ribosylation Ecto-ADP-ribosyltransferases ARTC SDS-PAGE autoradiography Flow cytometry Near-infrared in vivo imaging 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Stephan Menzel
    • 1
  • Sahil Adriouch
    • 1
    • 2
  • Peter Bannas
    • 1
    • 3
  • Friedrich Haag
    • 1
  • Friedrich Koch-Nolte
    • 1
  1. 1.Institute of ImmunologyUniversity Medical Center Hamburg-EppendorfHamburgGermany
  2. 2.Faculty of MedicineUniversity of RouenRouenFrance
  3. 3.Department of RadiologyUniversity Medical Center Hamburg-EppendorfHamburgGermany

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