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Differentiation of Human Pluripotent Stem Cells to Megakaryocytes by Transcription Factor-Driven Forward Programming

  • Thomas MoreauEmail author
  • Amanda L. Evans
  • Cedric J. G. GhevaertEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1812)

Abstract

The differentiation of megakaryocytes from human pluripotent stem cells in vitro offers intriguing new perspectives for research and transfusion medicine. However, applications have been hampered by the low efficiency of cytokine driven differentiation protocols leading to poor megakaryocyte purity and yield. Here we describe a novel forward programming approach relying on the combined ectopic expression of the three transcription factors GATA1, FLI1, and TAL1 in human pluripotent stem cells for large scale production of mature megakaryocytes using chemically defined culture and minimum cytokines.

Key words

Human pluripotent stem cells Megakaryocytes Transcription factors Forward programming Transfusion medicine 

Notes

Acknowledgments

This work was supported by NIHR, NHSBT, MRC grants and a core support grant from the Wellcome Trust and MRC to the Wellcome Trust – Medical Research Council Cambridge Stem Cell Institute.

Supplementary material

Supplementary video 1:

(MP4 46556 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Haematology, Wellcome Trust – Medical Research Council Cambridge Stem Cell InstituteNHS Blood and Transplant, Long Road, University of CambridgeCambridgeUK

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