Searching the GPCR Heterodimer Network (GPCR-hetnet) Database for Information to Deduce the Receptor–Receptor Interface and Its Role in the Integration of Receptor Heterodimer Functions

  • Ismel Brito
  • Manuel Narvaez
  • David Savelli
  • Kirill Shumilov
  • Michael Di Palma
  • Stefano Sartini
  • Kamila Skieterska
  • Kathleen Van Craenenbroeck
  • Ismael Valladolid-Acebes
  • Rauner Zaldivar-Oro
  • Malgorzata Filip
  • Riccardo Cuppini
  • Alicia Rivera
  • Fang Liu
  • Patrizia Ambrogini
  • Miguel Pérez de la Mora
  • Kjell Fuxe
  • Dasiel O. Borroto-Escuela
Part of the Neuromethods book series (NM, volume 140)


The G protein-coupled receptor heterocomplex network database (GPCR-hetnet) is a database designed to store information on GPCR heteroreceptor complexes and their allosteric receptor–receptor interactions. It is an expert-authored and peer-reviewed, curated collection of well-documented GPCR–GPCR interactions that span the gamut from classical GPCR–GPCR interactions to more complex receptor–receptor interactions (GPCR-Receptor Tyrosine Kinase and GPCR-ionotropic receptor/ligand gated ion channel). Although GPCR-hetnet contains interactions among GPCR from several different species, the curators have initially focused on receptor–receptor interactions in humans. Currently (August 2017) GPCR-hetnet contains information on 250 receptors (192 GPCR, 52 RTK, and 6 ionotropic receptors) and >1023 interactions. The GPCR-hetnet provides four searchable datasets: the hetnet, the non-hetnet, the rtknet, and the ionnet. Other supporting datasets include information about receptors that are present in GPCR-hetnet such as literature citations. This chapter describes in a basic protocol how to use, navigate, and browse through the GPCR-hetnet database to identify the clusters in which a receptor protomer of interest is involved, while further applicability are also described and introduced.

Key words

G protein-coupled receptors Ionotropic receptor/ligand gated ion channel Receptor tyrosine kinase Network Heterodimerization Heteromers Dimerization Oligomerization Hubs Receptor–receptor interactions Clusters Architecture 



The work was supported by the Swedish Medical Research Council (62X-00715-50-3) to K.F., by ParkinsonFonden 2015, AFA Försäkring (130328) to K.F., and by Hjärnfonden (FO2016-0302) and Karolinska Institutet Forskningsstiftelser (2016–2017) to D.O.B-E. D.O.B-E. belongs to the “Academia de Biólogos Cubanos” group.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Ismel Brito
    • 1
    • 2
  • Manuel Narvaez
    • 3
  • David Savelli
    • 4
  • Kirill Shumilov
    • 5
  • Michael Di Palma
    • 4
  • Stefano Sartini
    • 4
  • Kamila Skieterska
    • 6
  • Kathleen Van Craenenbroeck
    • 6
  • Ismael Valladolid-Acebes
    • 7
  • Rauner Zaldivar-Oro
    • 8
  • Malgorzata Filip
    • 9
  • Riccardo Cuppini
    • 4
  • Alicia Rivera
    • 5
  • Fang Liu
    • 10
  • Patrizia Ambrogini
    • 4
  • Miguel Pérez de la Mora
    • 11
  • Kjell Fuxe
    • 12
  • Dasiel O. Borroto-Escuela
    • 1
    • 2
    • 4
  1. 1.Department of NeuroscienceKarolinska InstitutetStockholmSweden
  2. 2.Observatorio Cubano de NeurocienciasGrupo Bohío-EstudioYaguajayCuba
  3. 3.Facultad de Medicina, Instituto de Investigación Biomédica de MálagaUniversidad de MálagaMálagaSpain
  4. 4.Department of Biomolecular Science, Section of PhysiologyUniversity of UrbinoUrbinoItaly
  5. 5.Departamento de Biología CelularUniversidad de MálagaMálagaSpain
  6. 6.Laboratory of Eukaryotic Gene Expression and Signal Transduction (LEGEST)Ghent UniversityGhentBelgium
  7. 7.The Rolf Luft Research Center for Diabetes and EndocrinologyKarolinska Institutet, Karolinska University Hospital L1StockholmSweden
  8. 8.Departamento de Bioquímica, Facultat de BiologíaUniversidad de la HabanaLa HabanaCuba
  9. 9.Laboratory of Drug Addiction Pharmacology, Institute of PharmacologyPolish Academy of SciencesKrakówPoland
  10. 10.Campbell Research Institute, Centre for Addiction and Mental HealthUniversity of TorontoTorontoCanada
  11. 11.Instituto de Fisiología CelularUniversidad Nacional Autónoma de MéxicoMexico CityMexico
  12. 12.Department of NeuroscienceKarolinska InstitutetStockholmSweden

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