Advertisement

Progranulin pp 289-304 | Cite as

Methods to Study the Role of Progranulin in Preimplantation Mouse Embryo Development

  • Laura Diaz-Cueto
  • Fabian Arechavaleta-Velasco
  • George L. Gerton
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1806)

Abstract

Progranulin is a 67–88 kDa glycoprotein, also known as acrogranin, PC-cell-derived growth factor, granulin-epithelin precursor, and proepithelin. This protein is present in a variety of mouse, rat, and human tissues. Progranulin, which is a growth factor, mediates cell cycle progression and cell migration in normal and pathological conditions. In several types of cancers, progranulin expression is upregulated, whereas function-interfering mutations in the granulin gene in humans have been linked to a subset of heritable cases of frontotemporal lobar degeneration. Also, progranulin has important effects on mouse preimplantation embryo development in vitro, including regulation of the appearance of the epithelium in the developing mouse blastocyst and growth of trophectoderm. Furthermore, progranulin promotes mouse blastocyst hatching, adhesion, and outgrowth in vitro. In this chapter, we describe some of the techniques that may be useful in the study of progranulin in embryo development.

Key words

Progranulin Embryo development 

Notes

Acknowledgment

The authors thank Manuel Mendez Montes for his assistance with figure preparation.

References

  1. 1.
    Fleming TP, Johnson MH (1988) From egg to epithelium. Annu Rev Cell Biol 4:459–485CrossRefPubMedGoogle Scholar
  2. 2.
    Diaz-Cueto L, Gerton GL (2001) The influence of growth factors on the development of preimplantation mammalian embryos. Arch Med Res 32:619–626CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Kaye PL (1997) Preimplantation growth factor physiology. Rev Reprod 2:121–127CrossRefPubMedGoogle Scholar
  4. 4.
    Diaz-Cueto L, Stein P, Jacobs A et al (2000) Modulation of mouse preimplantation embryo development by acrogranin (epithelin/granulin precursor). Dev Biol 217:406–418CrossRefPubMedGoogle Scholar
  5. 5.
    Qin J, Diaz-Cueto L, Schwarze JE et al (2005) Effects of progranulin on blastocyst hatching and subsequent adhesion and outgrowth in the mouse. Biol Reprod 73:434–442CrossRefPubMedGoogle Scholar
  6. 6.
    Handyside AH, Hunter S (1984) A rapid procedure for visualising the inner cell mass and trophectoderm nuclei of mouse blastocysts in situ using polynucleotide-specific fluorochromes. J Exp Zool 231:429–434CrossRefPubMedGoogle Scholar
  7. 7.
    Brison DR, Schultz RM (1997) Apoptosis during mouse blastocyst formation: evidence for a role for survival factors including transforming growth factor alpha. Biol Reprod 56:1088–1096CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Laura Diaz-Cueto
    • 1
  • Fabian Arechavaleta-Velasco
    • 1
  • George L. Gerton
    • 2
  1. 1.Research Unit in Reproductive MedicineUMAE Hospital de Gineco Obstetricia No. 4 “Luis Castelazo Ayala”, Instituto Mexicano del Seguro SocialMexico CityMexico
  2. 2.Center for Research on Reproduction and Women’s HealthPerelman School of Medicine, University of PennsylvaniaPhiladelphiaUSA

Personalised recommendations