Genipin Cross-Linking of Elastin and Elastin-Based Proteins
Genipin is a natural plant-derived compound that covalently cross-links biopolymers into lattice networks with good biocompatibility, controllable swelling, and mechanical properties. This protocol describes the genipin cross-linking of elastic proteins, including tropoelastin and elastin-based polypeptides, through steps of elastin phase-separation upon addition of salt and heat, centrifugation to rapidly concentrate the dense protein phase, and incubation. This method is applicable for the fabrication of elastic materials suitable for use as scaffolds for biomedical applications.
Key wordsGenipin Elastin Elastin-like polypeptide Cross-link Protein scaffold Hydrogel
The author thanks Fred Keeley and Sean Reichheld for useful discussions.