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Chick Embryonic Cardiomyocyte Micromass System for Assessing Developmental Cardiotoxicity of Drugs

  • Wasay Mohiuddin Shaikh Qureshi
  • Margaret K. Pratten
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1797)

Abstract

Heart is the first mesodermal organ to develop and is sensitive to life-threatening toxic effects of drugs during development. A number of methods have been devised to study developmental cardiotoxic effects of drugs including micromass system. The micromass system involves the culture of primary embryonic cells and reestablishment of tissue system in vitro. In chick embryonic cardiomyocyte micromass system the chick heart cells are cultured in a small volume at a very high cell density. These cells form synchronized contracting foci. Addition of drugs to this system allows us to study the developmental cardiotoxic effects at molecular level. Using appropriate end points and molecular marker or adopting high-throughput screening, this method can further help to identify and avoid the use of cardiotoxic compounds during development.

Key words

Developmental toxicology Micromass Cardiomyocytes Teratogens Chick Reactive oxygen species Connexin43 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Wasay Mohiuddin Shaikh Qureshi
    • 1
    • 2
  • Margaret K. Pratten
    • 1
  1. 1.School of Life SciencesUniversity of Nottingham, Queen’s Medical CentreNottinghamUK
  2. 2.Cardiovascular Research Centre, Institute of Genetic MedicineNewcastle UniversityNewcastle upon TyneUK

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