Using Yeast Hybrid System to Identify Proteins Binding to Small Molecules

  • You Wang
  • David S. Letham
  • Peter C. L. John
  • Ren ZhangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1794)


Protein–small molecule interaction studies provide useful insights into biological processes taking place within the living cell. A special yeast hybrid system, the yeast three-hybrid method, has been developed and used to explore proteins that bind to small molecules, by which means it may be possible to unravel biological processes and dissect function of biological systems. Here we present a protocol employing this method for identifying such binding proteins.

Key words

Yeast three-hybrid system Yeast two-hybrid system Protein–small molecule interactions 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • You Wang
    • 1
  • David S. Letham
    • 2
  • Peter C. L. John
    • 2
  • Ren Zhang
    • 1
    Email author
  1. 1.School of Biological SciencesUniversity of WollongongWollongongAustralia
  2. 2.Research School of BiologyAustralian National UniversityCanberraAustralia

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