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RNA-Sequencing from Low-Input Material in Multiple Myeloma for Application in Clinical Routine

  • Anja Seckinger
  • Tomi Bähr-Ivacevic
  • Vladimir Benes
  • Dirk Hose
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1792)

Abstract

RNA sequencing is a recently developed approach for transcriptome profiling with several advantages over gene expression profiling using DNA microarrays. Here we describe a RNA-sequencing protocol optimized for low-input analysis of total RNA from CD138+ purified plasma cells from myeloma patients which is applicable in clinical routine.

Key words

Multiple myeloma RNA-sequencing Low input Clinical application 

Notes

Acknowledgments

This work was in part supported by grants from the German Federal Ministry of Education (BMBF) within the framework of the e:Med research and funding concept “CLIOMMICS” (01ZX1309 and 01ZX1609) and the Deutsche Forschungsgemeinschaft (DFG SFB/TRR79; TP B1 and B12).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Anja Seckinger
    • 1
  • Tomi Bähr-Ivacevic
    • 1
  • Vladimir Benes
    • 2
  • Dirk Hose
    • 1
  1. 1.Labor für Myelomforschung, Medizinische Klinik VUniversitätsklinikum HeidelbergHeidelbergGermany
  2. 2.Genomics Core Facility, European Molecular Biology LaboratoryHeidelbergGermany

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