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Deep Profiling of the Immune System of Multiple Myeloma Patients Using Cytometry by Time-of-Flight (CyTOF)

  • Tina Smets
  • Frederik Stevenaert
  • Homer AdamsIII
  • Greet Vanhoof
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1792)

Abstract

Mass cytometry has emerged as a new state-of-the-art technology that enables in-depth characterization of cellular populations and functions at a single cell resolution. We describe the application of this technology to deeply phenotype the blood and bone marrow components of multiple myeloma patients in a clinical setting. This technology allows for simultaneous quantification of more than 40 markers, overcoming the challenges of traditional fluorescence-based flow cytometry.

Key words

Mass cytometry CyTOF Multiple myeloma Immunophenotyping Single cell Barcoding Smart tube Peripheral blood Bone marrow 

Notes

Acknowledgments

The authors would like to thank Christoph Heuck for his suggestions and critical reading of the manuscript.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Tina Smets
    • 1
  • Frederik Stevenaert
    • 1
  • Homer AdamsIII
    • 2
  • Greet Vanhoof
    • 1
  1. 1.Janssen Research and DevelopmentBeerseBelgium
  2. 2.Janssen Research and Development, LLCSpring HouseUSA

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