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Myelin pp 67-77 | Cite as

Proteomics Analysis of Myelin Composition

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1791)

Abstract

The identification and functional characterization of the repertoire of myelin proteins provides a valuable foundation for understanding molecular mechanisms of myelination and the pathogenesis of human myelin disease. Here we provide a procedure for the purification of myelin from rodent or human brains and a large-scale analysis of the myelin proteome, using the shotgun approach of one-dimensional PAGE and liquid chromatography (LC)/tandem mass spectrometry (MS).

Key words

Myelin Proteomics Tandem mass spectrometry Liquid chromatography Sucrose gradient 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of NeuroscienceUniversity of Connecticut Medical SchoolFarmingtonUSA

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