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Myelin pp 25-35 | Cite as

Visualization and Time-Lapse Microscopy of Myelinating Glia In Vivo in Zebrafish

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1791)

Abstract

In vivo time-lapse microscopy provides important information about the kinetics of cellular events and their control by interactions with neighboring cells. Here, we describe the generation and use of transgenic zebrafish to visualize dynamics of myelinating glia using cell type-specific expression and microscopy of genetically encoded fluorescent proteins. With this method, we are able to simultaneously separate and trace up to three different colors over time.

Key words

Oligodendrocyte Myelin In vivo imaging Central nervous system 

Notes

Acknowledgments

Our group is supported by an Emmy Noether fellowship of the German Research Foundation (DFG, Cz226/1-1), a Starting Grant of the European Research Council (ERC-StG 714440, MecMy) and the Munich Cluster of Systems Neurology (SyNergy, DFG, EXC1010).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Neuronal Cell Biology, Technical University of MunichMunichGermany
  2. 2.Munich Cluster of Systems Neurology (SyNergy)MunichGermany

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