Visualization and Time-Lapse Microscopy of Myelinating Glia In Vivo in Zebrafish
In vivo time-lapse microscopy provides important information about the kinetics of cellular events and their control by interactions with neighboring cells. Here, we describe the generation and use of transgenic zebrafish to visualize dynamics of myelinating glia using cell type-specific expression and microscopy of genetically encoded fluorescent proteins. With this method, we are able to simultaneously separate and trace up to three different colors over time.
Key wordsOligodendrocyte Myelin In vivo imaging Central nervous system
Our group is supported by an Emmy Noether fellowship of the German Research Foundation (DFG, Cz226/1-1), a Starting Grant of the European Research Council (ERC-StG 714440, MecMy) and the Munich Cluster of Systems Neurology (SyNergy, DFG, EXC1010).