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Myelin pp 3-13 | Cite as

Electron Microscopy for the Analysis of Peripheral Nerve Myelin

  • Cinzia Ferri
  • Angelo Quattrini
  • Maurizio D’Antonio
Part of the Methods in Molecular Biology book series (MIMB, volume 1791)

Abstract

Transmission electron microscopy (TEM) can provide excellent high-resolution images of cellular structures. A critical step with this technique is sample preparation. Here we describe our protocol for the preparation of semithin and ultrathin sections of mouse peripheral nerves in order to visualize the structure and pathological features of peripheral myelin with TEM.

Key words

Myelin Schwann cell Periodicity TEM Semithin sectioning Ultrathin sectioning Sample preparation 

Notes

Acknowledgments

This work was supported by Fondazione Telethon (GGP14147) and the Italian Ministry of Health (GR-2011-0234679).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Cinzia Ferri
    • 1
  • Angelo Quattrini
    • 2
  • Maurizio D’Antonio
    • 1
  1. 1.Division of Genetics and Cell BiologySan Raffaele Scientific InstituteMilanItaly
  2. 2.Division of NeuroscienceSan Raffaele Scientific InstituteMilanItaly

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