Optical Bioluminescence Protocol for Imaging Mice

  • David Stout
  • John David
Part of the Methods in Molecular Biology book series (MIMB, volume 1790)


The presence, growth, or decline of transfected cell populations expressing the enzyme Luciferase can be followed in live mice using bioluminescence optical imaging techniques. This protocol describes how to verify the imaging equipment, options for injecting the substrate Luciferin into mice, image acquisition considerations, and commonly used data analysis techniques.

Key words

Bioluminescence Luciferin Luciferase Optical imaging Mouse Oncology Tumor imaging 



This work was made possible by numerous faculty, student, and staff members at the UCLA Crump Institute for Molecular Imaging Preclinical Technology Imaging Center. Waldemar Ladno, DVM and Darin Williams were invaluable with veterinary and logistical help with operations, education, and research work.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • David Stout
    • 1
  • John David
    • 2
  1. 1.D&D DesignCulver CityUSA
  2. 2.PfizerLa JollaUSA

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