Vacuolar Targeting and Characterization of Recombinant Antibodies
Plant-based platforms are extensively use for the expression of recombinant proteins, including monoclonal antibodies (mAbs). Generally, immunoglobulins (Igs) are sorted to the apoplast, which is often afflicted with intense proteolysis. Here, we describe methods to transiently express mAbs sorted to central vacuole in Nicotiana benthamiana leaves and to characterize the obtained IgG. Central vacuole is an appropriate compartment for the efficient production of Abs, consequently vacuolar sorting should be considered as an alternative strategy to obtain high protein yields.
Key wordsImmunoglobulin N-glycosylation Vacuolar sorting signals Secretory pathway Vacuolar transport Molecular farming
This work was supported by the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) through the grants PICT Start Up 2015-0010 and PICT 2016-1722 and by Universidad Nacional de La Plata (grant 11X/754) to S.P. S.P. is a researcher from CONICET and Full Professor of the Facultad de Ciencias Exactas-UNLP; CGO is a fellow at CONICET.
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