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Macrophages pp 87-92 | Cite as

Isolation and Phenotyping of Bone Marrow Macrophages

  • Marion Chalot
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1784)

Abstract

Macrophages are present in most of the tissues in the organism. They are basically separated into two categories: the resident macrophages, specific of the tissue and capable of proliferation, and the macrophages deriving from the monocyte differentiation. In the bone marrow, the “resident” macrophages are part of the hematopoietic stem cell niche.

Those macrophages are known to have a role in the support of erythropoiesis (Chow, Nat Med 19:429–436, 2013), the maintenance of stem cell in their niches (Chow, J Exp Med 208:261–271, 2011), and are an independent self-renewing population (Hashimoto, Immunity 38:792–804, 2013).

Key words

“Resident” macrophages Bone marrow Cell sorting Cell culture 

References

  1. 1.
    Chow A, Lucas D, Hidalgo A et al (2011) Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche. J Exp Med 208:261–271CrossRefPubMedPubMedCentralGoogle Scholar
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    Christopher MJ, Rao M, Liu F et al (2011) Expression of the G-CSF receptor in monocytic cells is sufficient to mediate hematopoietic progenitor mobilization by G-CSF in mice. J Exp Med 208:251–260CrossRefPubMedPubMedCentralGoogle Scholar
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    Winkler IG, Sims NA, Pettit AR et al (2010) Bone marrow macrophages maintain hematopoietic stem cells (HSC) niches and their depletion mobilizes HSCs. Blood 116:4815–4828CrossRefPubMedGoogle Scholar
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    Ehninger A, Trumpp A (2011) The bone marrow stem cell niche grows up: mesenchymal stem cells and macrophages move in. J Exp Med 208:421–428CrossRefPubMedPubMedCentralGoogle Scholar
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    Chow A, Huggins M, Ahmed J et al (2013) CD169+ macrophages provide a niche promoting erythropoiesis under homeostasis and stress. Nat Med 19:429–436CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Hashimoto D, Chow A, Noizat C et al (2013) Tissue-resident macrophages self-maintain locally throughout adult life with minimal contribution from circulating monocytes. Immunity 38:792–804CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Marion Chalot
    • 1
    • 2
    • 3
    • 4
  1. 1.CEA/DRF/IBFJ/iRCM/LRTS, INSERM U967Fontenay aux RosesFrance
  2. 2.Université Paris-DiderotParisFrance
  3. 3.Université Paris-SudOrsayFrance
  4. 4.Equipe labellisée Ligue contre le CancerFontenay aux RosesFrance

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