Nature has designed nanosized particles, specifically viruses, equipped to deliver cargo to cells. We report the chemical bioconjugation and shape shifting of a hollow, rod-shaped tobacco mosaic virus (TMV) to dense spherical nanoparticles (SNPs). We describe methods to transform TMV rods to spheres, load TMV rods and spheres with the chemotherapeutic drug, doxorubicin (DOX), to deliver modified particles to breast cancer cells, and to determine the IC50 values of the plant virus-based drug delivery system.
Tobacco mosaic virus (TMV) Viral nanoparticles (VNPs) Bioconjugation Doxorubicin (DOX) Drug delivery Breast cancer
This is a preview of subscription content, log in to check access.
Springer Nature is developing a new tool to find and evaluate Protocols. Learn more
This work was supported in part by a grant from the National Science Foundation (DMR 1452257 to N.F.S.).
Group, U.S.C.S.W. 2015. vol. 2016 (U.S. Department of Health and Human Services, Centers for Disease Control and Prevention and National Cancer Institute)Google Scholar
Marchal S, Hor AE, Millard M, Gillon V, Bezdetnaya L (2015) Anticancer drug delivery: an update on clinically applied nanotherapeutics. Drugs 75:1601–1611CrossRefPubMedGoogle Scholar
Bruckman MA et al (2014) Biodistribution, pharmacokinetics, and blood compatibility of native and PEGylated tobacco mosaic virus nano-rods and -spheres in mice. Virology 449:163–173CrossRefPubMedGoogle Scholar
Wen AM, Le N, Zhou X, Steinmetz NF, Popkin DL (2015) Tropism of CPMV to professional antigen presenting cells enables a platform to eliminate chronic infections. ACS Biomaterials Science & Engineering 1:1050–1054CrossRefGoogle Scholar
Bromley KM, Patil AJ, Perriman AW, Stubbs G, Mann S (2008) Preparation of high quality nanowires by tobacco mosaic virus templating of gold nanoparticles. Journal of Materials Chemistry 18:4796–4801CrossRefGoogle Scholar
Miller RA, Presley AD, Francis MB (2007) Self-assembling light-harvesting systems from synthetically modified tobacco mosaic virus coat proteins. Journal of the American Chemical Society 129:3104–3109CrossRefPubMedGoogle Scholar
Bruckman MA, Czapar AE, VanMeter A, Randolph LN, Steinmetz NF (2016) Tobacco mosaic virus-based protein nanoparticles and nanorods for chemotherapy delivery targeting breast cancer. J Control Release 231:103CrossRefPubMedPubMedCentralGoogle Scholar
Bruckman MA, VanMeter A, Steinmetz NF (2015) Nanomanufacturing of tobacco mosaic virus-based spherical biomaterials using a continuous flow method. ACS Biomater Sci Eng 1:13–18CrossRefPubMedGoogle Scholar
Czapar AE, Steinmetz NF (2016) In: Lu Z-R, Sakuma S (eds) Nanomaterials in pharmacology. Springer, New York, NY, pp 65–85CrossRefGoogle Scholar