Artificial Microniche Array with Spatially Structured Biochemical Cues

  • Xumei Gao
  • Celine Stoecklin
  • Yue Zhang
  • Zhe Weng
  • Richard De Mets
  • Gianluca Grenci
  • Virgile Viasnoff
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1771)

Abstract

We present here a method to create arrays of microcavities that can be differentially coated on their bottom, side, and top with different proteins. These cavities range in size from single cell to multicellular aggregate. We provide detailed protocols to create such arrays with some variations using different materials and different coating proteins. The use of such cavities as bona fide artificial microniches to mimic cellular microenvironments has been already established and is referenced.

Key words

Array Microcavities Artificial microniche Cellular microenvironment 

Notes

Acknowledgments

The authors acknowledge financial support from the Mechanobiology Institute of Singapore and from CNRS (France).

References

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Xumei Gao
  • Celine Stoecklin
  • Yue Zhang
    • 1
  • Zhe Weng
    • 1
  • Richard De Mets
    • 1
  • Gianluca Grenci
    • 1
  • Virgile Viasnoff
    • 1
    • 2
    • 3
  1. 1.Mechanobiology InstituteNational University of SingaporeSingaporeSingapore
  2. 2.Centre National Pour la Recherche ScientifiqueUMI3639SingaporeSingapore
  3. 3.Department of Biological Sciences at NUSSingaporeSingapore

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