Micropropagation of Orchids by Using Bioreactor Technology
Orchids comprise an important group of flowering plants that produce a wide and distinct variety of flowers with characteristic shapes, size, colors, and fragrance. In horticulture industry, orchids are used as cut flowers and as potted plants. Many of the orchids have also seen medicinal use as part of traditional Chinese medicine. To meet the extensive demand for their attractive and showy flowers in the commercial market, tissue culture methods have been developed in many ornamental orchids for their large-scale multiplication. Recently, the bioreactor systems such as airlift, bubble, and temporary immersion bioreactors have been established for large-scale propagation of protocorm-like bodies (PLBs), shoots, and plantlets of various orchids. This chapter describes the bioreactor culture methods for micropropagation of Anoectochilus formosanus and Dendrobium candidum. Methods of estimation of total phenolics, flavonoids, polysaccharides, and antioxidant activities of biomass produced from Dendrobium candidum are also discussed.
Key wordsAnoectochilus formosanus Bioreactor culture Dendrobium candidum Flavonoids Phenolics Polysaccharides Protocorm-like bodies
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