Protocols for Establishing Fungi-Protocorm Culture

  • Yuan-Yuan Li
  • Hui Wang
  • Shun-Xing Guo
Part of the Springer Protocols Handbooks book series (SPH)


Establishing fungi-protocorm culture requires the isolation, identification, and in vitro germination test of symbiotic fungi. In situ seed baiting is a useful technique to attract and isolate seed germination-promoting fungi. In this chapter, a protocol for establishing protocorm-fungi culture using a seed baiting technique is detailed. This technique enables the identification of potential mycorrhizal fungi specifically at the time of seed germination and during protocorm development.

Key words

Seed baiting Symbiotic germination Protocorm Mycorrhizal fungi Orchid 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Yuan-Yuan Li
    • 1
  • Hui Wang
    • 1
  • Shun-Xing Guo
    • 1
  1. 1.Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingPeople’s Republic of China

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