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New Tools for Imaging of Immune Systems: Visualization of Cell Cycle, Cell Death, and Cell Movement by Using the Mice Lines Expressing Fucci, SCAT3.1, and Kaede and KikGR

  • Michio Tomura
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1763)

Abstract

Visualization of biological events in real time in vivo has become a crucial to understand immune responses. We have been established novel visualization tools for life of immune cells: proliferation, cell death, and migration. Fucci-transgenic mice allow us to visualize cell cycle phases by reciprocal expression of mKusabira-Orange2 in G1 phase and mAzami-Green in S/G2/M phase. Caspase-3 indicator SCAT3.1 knock-in mice visualize cell death by changing color. Photoconvertible proteins, Kaede and KikGR expressing mice track cell movement between organs by labeling immune cells as red color. Here, I will introduce how to use and visualize these mice. These techniques will help to understand immune system in the living whole body.

Key words

Cell cycle Fucci Photoconvertible protein Cell migration Kaede KikGR Cell death Caspase-3 SCAT3.1 

Notes

Acknowledgements

This work was supported in part by JSPS Grants-in-Aid for Scientific Research in Innovative Areas “Analysis and Synthesis of Multidimensional Immune Organ Network” (#24111007); JSPS Grants-in-Aid for Scientific Research (B) (#16H05087); Special Coordination Funds for Promoting Science and Technology of the Japanese Government.

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Michio Tomura
    • 1
  1. 1.Laboratory of Immunology, Faculty of PharmacyOsaka Ohtani UniversityOsakaJapan

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