Characterizing Intact Macromolecular Complexes Using Native Mass Spectrometry

  • Elisabetta Boeri Erba
  • Luca Signor
  • Mizar F. Oliva
  • Fabienne Hans
  • Carlo Petosa
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1764)

Abstract

Native mass spectrometry (MS) enables the characterization of macromolecular assemblies with high sensitivity. It can reveal the stoichiometry of subunits as well as their two-dimensional interaction network and provide information regarding the dynamic behavior of macromolecular complexes. Here, we describe the workflow to perform native MS experiments. In addition, we illustrate the quality control analysis of proteins using MS in denaturing conditions.

Key words

Macromolecular assemblies Native mass spectrometry Stoichiometry Two-dimensional map of interactions 
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Notes

Acknowledgment

We thank the members of the Viral Infection and Cancer Group at the IBS for the helpful discussion. This work used the mass spectrometry platform of the Grenoble Instruct Centre (ISBG; UMS 3518 CNRS-CEA-UJF-EMBL) with support from FRISBI (ANR-10-INSB-05-02) and GRAL (ANR-10-LABX-49-01) within the Grenoble Partnership for Structural Biology (PSB). It was financially supported by the French Infrastructure for Integrated Structural Biology Initiative and by the French National Centre for Scientific Research (CNRS).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Elisabetta Boeri Erba
    • 1
  • Luca Signor
    • 1
  • Mizar F. Oliva
    • 1
  • Fabienne Hans
    • 1
  • Carlo Petosa
    • 1
  1. 1.Institut de Biologie Structurale (IBS)Université de Grenoble Alpes, CEA, CNRSGrenobleFrance

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