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An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome

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Next Generation Sequencing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1712))

Abstract

Recent advances in global translatome analysis technologies enable us to understand how translational regulation of gene expression modulates cellular functions. In this chapter, we present an integrated method to measure various aspects of translatome by polysome profiling and ribosome profiling using purified B cells. We standardized our protocols to directly compare the results from these two approaches. Parallel assessment of translatome with these two approaches can generate a comprehensive picture on how translational regulation determines protein output.

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Acknowledgments

We thank Jovan Shepherd for critical reading of manuscript. C.X. is a Pew Scholar in Biomedical Sciences. This study is supported by the PEW Charitable Trusts, Cancer Research Institute, National Institute of Health (R01AI087634, R01AI089854, RC1CA146299, R56AI110403, and R01AI121155 to C.X.).

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Authors and Affiliations

  1. Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 North Torrey Pines Road, R202, La Jolla, CA, 92037, USA

    Hyun Yong Jin & Changchun Xiao

Authors
  1. Hyun Yong Jin
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  2. Changchun Xiao
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Corresponding author

Correspondence to Changchun Xiao .

Editor information

Editors and Affiliations

  1. Next Generation Sequencing and Microarray Core Facility, The Scripps Research Institute, La Jolla, California, USA

    Steven R. Head

  2. Next Generation Sequencing and Microarray Core Facility, The Scripps Research Institute, La Jolla, California, USA

    Phillip Ordoukhanian

  3. Department of Molecular Medicine, The Scripps Research Institute Center for Organ and Cell Transplantation, La Jolla, California, USA

    Daniel R. Salomon

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Jin, H.Y., Xiao, C. (2018). An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome. In: Head, S., Ordoukhanian, P., Salomon, D. (eds) Next Generation Sequencing. Methods in Molecular Biology, vol 1712. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7514-3_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7514-3_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7512-9

  • Online ISBN: 978-1-4939-7514-3

  • eBook Packages: Springer Protocols

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