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In Vivo Electrochemical Studies of Optogenetic Control of Glutamate Signaling Measured Using Enzyme-Based Ceramic Microelectrode Arrays

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Biochemical Approaches for Glutamatergic Neurotransmission

Part of the book series: Neuromethods ((NM,volume 130))

Abstract

Direct electrochemical measurements of glutamate release in vivo were combined with optogenetics in order to examine light-induced control of glutamate neurotransmission in the rodent brain. Self-referenced recordings of glutamate using ceramic-based microelectrode arrays (MEAs) in hippocampus and frontal cortex demonstrated precise optical control of light-induced glutamate release through channelrhodopsin (ChR2) expression in both rat hippocampus and frontal cortex. Although the virus was only injected unilaterally, bilateral and rostro-caudal expression was observed in slice imaging, indicating diffusion and active transport of the viral particles. Methodology for the optogenetic control of glutamate signaling in the rat brain is thoroughly explained with special attention paid to MEA enzyme coating and cleaning for the benefit of other investigators. These data support that optogenetic control of glutamate signaling is robust with certain advantages as compared to other methods to modulate the in vivo control of glutamate signaling.

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Acknowledgments

Supported by NIDA; R21DA033796-01, DARPA; N66001-09-C-2080 and NIH; CTSA 1 UL1RR033173-01. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.

Disclosure of competing interest: G.A.G. is principal owner of Quanteon LLC. J.E.Q., P.H., and F.P. have served as consultants to Quanteon LLC.

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Authors and Affiliations

  1. Department of Anatomy and Neurobiology, University of Kentucky Medical Center, Lexington, 40536, KY, USA

    Jason J. Burmeister, Francois Pomerleau, Jorge E. Quintero, Peter Huettl, Yi Ai, John T. Slevin & Greg A. Gerhardt

  2. Lund University, Lund, Sweden

    Johan Jakobsson, Martin Lundblad & Andreas Heuer

Authors
  1. Jason J. Burmeister
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  2. Francois Pomerleau
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  3. Jorge E. Quintero
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  4. Peter Huettl
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  5. Yi Ai
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  6. Johan Jakobsson
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  7. Martin Lundblad
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  8. Andreas Heuer
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  9. John T. Slevin
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  10. Greg A. Gerhardt
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Corresponding author

Correspondence to Greg A. Gerhardt .

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Editors and Affiliations

  1. Lyon Neuroscience Research Center, University Lyon 1, Lyon, France

    Sandrine Parrot

  2. Lyon Neuroscience Research Center, University Lyon 1, Lyon, France

    Luc Denoroy

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Burmeister, J.J. et al. (2018). In Vivo Electrochemical Studies of Optogenetic Control of Glutamate Signaling Measured Using Enzyme-Based Ceramic Microelectrode Arrays. In: Parrot, S., Denoroy, L. (eds) Biochemical Approaches for Glutamatergic Neurotransmission. Neuromethods, vol 130. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7228-9_11

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  • DOI: https://doi.org/10.1007/978-1-4939-7228-9_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7227-2

  • Online ISBN: 978-1-4939-7228-9

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