Erratum to: Effector Translocation: Cya Reporter Assay

  • Suma Chakravarthy
  • Bethany Huot
  • Brian H. KvitkoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1615)

Erratum to: Chapter 33 in: Laure Journet and Eric Cascales (eds.), Bacterial Protein Secretion Systems: Methods and Protocols, Methods in Molecular Biology, vol. 1615,

An error was observed in Step 2 of section 3.3. Direct cAMP Assay of this chapter. The same was replaced with the correction below.

2. Vortex the frozen tubes rapidly so the copper BBs grind the tissue into a fine powder. Keep the tubes with powdered plant material frozen in liquid nitrogen until immediately prior to adding 0.1 M HCl in Step 3. Ground tissue can also be frozen at −80°C until use. (See Note 14).

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© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Suma Chakravarthy
    • 1
  • Bethany Huot
    • 2
  • Brian H. Kvitko
    • 3
    Email author
  1. 1.Plant Pathology and Plant-Microbe Biology SectionSchool of Integrative Plant Science, Cornell UniversityIthacaUSA
  2. 2.Department of Energy, Plant Research LaboratoryMichigan State UniversityEast LansingUSA
  3. 3.Department of Plant PathologyUniversity of GeorgiaAthensUSA

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