Constructing Synthetic Microbial Communities to Explore the Ecology and Evolution of Symbiosis
Synthetically engineered microbial communities based on model organisms provide a simplified model of their naturally occurring counterparts while still retaining essential features of living organisms. The degree of control afforded by this approach has been critical in understanding how similar types of natural communities might have persisted and evolved. Here, we first discuss important considerations when designing a synthetically engineered system. Then, we describe the steps required to create a two-partner cooperative system based on the yeast Saccharomyces cerevisiae.
Key wordsEvolution Ecology Mutualism Cooperation Synthetic biology S. cerevisiae
Work in the W.S. group is supported by the W. M. Keck Foundation and the National Institutes of Health (Grant 1 DP2OD006498-01).
- 7.SGD project Saccharomyces Genome Database. In: SGD. http://yeastgenome.org/cache/genomeSnapshot.html. Accessed 8 Mar 2010
- 19.Guthrie C, Fink GR (2002) Guide to yeast genetics and molecular and cell biology, part B, vol 350, 1st edn. Academic, New YorkGoogle Scholar
- 22.Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar