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Constructing Synthetic Microbial Communities to Explore the Ecology and Evolution of Symbiosis

  • Adam James Waite
  • Wenying Shou
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1151)

Abstract

Synthetically engineered microbial communities based on model organisms provide a simplified model of their naturally occurring counterparts while still retaining essential features of living organisms. The degree of control afforded by this approach has been critical in understanding how similar types of natural communities might have persisted and evolved. Here, we first discuss important considerations when designing a synthetically engineered system. Then, we describe the steps required to create a two-partner cooperative system based on the yeast Saccharomyces cerevisiae.

Key words

Evolution Ecology Mutualism Cooperation Synthetic biology S. cerevisiae 

Notes

Acknowledgements

Work in the W.S. group is supported by the W. M. Keck Foundation and the National Institutes of Health (Grant 1 DP2OD006498-01).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Molecular and Cellular Biology ProgramUniversity of WashingtonSeattleUSA
  2. 2.Division of Basic SciencesFred Hutchinson Cancer Research CenterSeattleUSA

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